ABSTRACT
Objective To compare the effects of curcumin and enalapril on transdifferentiation of renal tubular epithelial cells in unilateral ureteral obstruction (UUO) rats. Method UUO rat model was used. Male wistar rats were randomly divided into four groups with 8 rats each:shame-operated group (0.9% saline solution), model group (dimethyl sulphoxide), curcumin treatment group [curcumin 100 mg/(kg?d)], and enalapril treatment group [enalapril 10 mg/(kg?d)]. Medicines were given intraperitoneally daily two days after the operation. All rats were killed 4w after surgery. The degree of tubulointerstitial fibrosis was scored by HE and Masson staining, and the sites and levels of protein expression of TGF-?1、?-SMA and vimentin were detected by immunohistochemistry staining. Result Compared with shame-operated group, the serum urea nitrogen level was significantly increased (P 0.05). Conclusion The pharmacological effects of curcumin and enalapril may be partly mediated by inhibiting the transdifferentiation of renal tubular epithelial cells towards myofibroblast in UUO rats.
ABSTRACT
Objective To investigate the effect of curcumin on renal interstitial fibrosis following unilateral ureteral obstruction (UUO), and compare the effects of Curcumin with that of Enalapril. Method UUO rat model was used. Male Wistar rats were randomly divided into four groups with 8 rats each:shame-operated group (0.9% saline solution), model group (dimethyl sulphoxide, DMSO), Curcumin treatment group [curcumin 100 mg/(kg?d)], and Enalapril treatment group [enalapril 10 mg/(kg?d)]. All rats were killed 4 w after surgery. The degree of tubulointerstitial fibrosis was scored by HE and Masson staining. The sites and levels of protein expression of TGF-?1, CTGF and TIMP-1 were detected by immunohistochemistry staining. Result Compared with those of shame-operated group, the blood urea nitrogen level was significantly increased (P 0.05). Conclusion Pharmacological effects of Curcumin or Enalapril on unilateral ureteral obstruction rats are quite similar. These effects may result from the down-regulation of TGF-?1, CTGF and TIMP-1 expression in kidney tissue.